Please use this identifier to cite or link to this item: http://hdl.handle.net/1946/11181
The effect of expanding mesenchymal stem cells in media supplemented with lysate manufactured from fresh or expired platelet concentrates on chondrogenic differentiation
Áhrif þess að fjölgamesenchymal stofnfrumum í æti með viðbættu lýsati, útbúnu úr ferskum eða útrunnum blóðflögum, á brjósksérhæfingu þeirra
Mesenchymal stem cells (MSCs) are a group of heterogeneous cells that can be isolated from numerous tissues, differentiated into various cell lines and expanded extensively in vitro. Additionally, they possess anti-inflammatory properties, therefore providing great potential for stem cell tissue engineering and regenerative medicine. Traditionally, foetal bovine serum (FBS) is used for in vitro culturing of MSCs but recently, alternatives such as human platelet derived culture mediums have been explored due to the potential harmful effect of using animal serum.
In this study we expanded the MSCs in lysates produces from fresh and expired human platelet rich concentrates (HPLF and HPLO respectively) as well as FBS for comparison. Once expanded, chondrogenisis was induced by an aggregate pellet culture in a chondrogenic medium for duration of 31 days. At days 14 and 31, pellets were harvested for a histochemistry assay, glycosaminoclycan assay and a real time quantitative polymerase chain reaction assay.
The MSCs expanded in HPLF displayed more favourable chondrogenic properties in camparison to the other two treatment mediums. This could potentially be caused by growth factors and cytokines present in human platelets, which may be of better quality in the HPLF compared with HPLO.