Please use this identifier to cite or link to this item: http://hdl.handle.net/1946/12410
TAP tagging proteins in Saccharomyces Cerevisiae
Tandem Affinity Purification (TAP) is a technique based on attaching a tag to the N- or C- terminus of target proteins. The tagged proteins can then be used for studies of protein-protein or protein-DNA interactions. In this study TAP tags of Med7, Tfb3, Spt3, Spt15 and Gal80 were attached to the C-termini in the yeast Saccharomyces cerevisiae by transformation with PCR-generated DNA fragments which integrate into the yeast genome by homologous recombination. The TAP tags will facilitate functional studies of these proteins in transcriptional regulation of the GAL network, and their interactions.