EnglishisÍslenska

Member institutions

Search in


ThesisUniversity of Iceland>Verkfræði- og náttúruvísindasvið>Meistaraprófsritgerðir>

Please use this identifier to cite or link to this item: http://hdl.handle.net/1946/13287

Titles
  • Characterization of trypsin forms from Atlantic cod (Gadus morhua)

  • is

    Greining á trypsín formum úr Atlantshafsþorski (Gadus morhua)

Published
October 2012
Abstracts
  • Trypsins from Atlantic cod (Gadus morhua) are highly active cold-adapted proteases. The
    purpose of this project was to characterize a benzamidine purified trypsin isolate from
    Atlantic cod with special emphasis on trypsin Y. Furthermore, the purpose was to compare
    the kinetic parameters (kcat, KM and kcat/KM) of cod trypsin Y and cod trypsin I towards
    various chromogenic substrates. The aim was also to compare the ability of cod trypsin and
    bovine trypsin to degrade native proteins. The results of this study showed that eight
    trypsins (trypsins 1-8) were present in the isolate based on 2D gel electrophoresis and
    cation exchange chromatography. Mass spectrometry analysis on trypsins 1-8 identified
    trypsin 1 as trypsin I, trypsins 2-7 as trypsin X and trypsin 8 as trypsin Y. The catalytic
    efficiency of trypsin I was found to be higher than that of trypsin Y towards chromogenic
    substrates at 25°C. The degradation ability of the cod trypsin isolate was 3-12 times higher
    than that of bovine trypsin depending on the substrate.

  • is

    Trypsín úr Atlantshafsþorski (Gadus morhua) eru mjög virkir, kulda-aðlagaðir próteinasar.
    Markmið verkefnis var að greina benzamidín hreinsaða trypsín blöndu úr Atlantshafsþorski
    með sérstakri áherslu á nýstárlegt form trypsíns (trypsín Y). Einnig, var markmið verkefnis
    að ákvarða og bera saman hraðafræðilega fasta (kcat, KM og kcat/KM) trypsíns I og trypsíns Y gegn mismunandi gerðum smárra litmyndandi hvarfefna. Þá var einnig borin saman geta þorskatrypsíns og nautatrypsíns við niðurbrot á próteinum í virkri myndbyggingu. Niðurstöður verkefnis sýna að 8 trypsín ísóform er að finna í trypsín blöndu út frá tvívíðum gel-rafdrætti og keyrslu á katjóna skilju. Massagreining á trypsínum 1-8 greinir trypsín 1 sem trypsín I, trypsín 2-7 sem trypsín X og trypsín 8 sem trypsín Y. Hvötunargeta trypsín I reyndist hærri en trypsíns Y við 25°C. Niðurbrotsgeta þorskatrypsín blöndu var allt að 12 sinnum hærri (fór eftir próteini) en nautatrypsíns.

Issued Date
12/10/2012


Artifacts
Name[Sortable]Size[Sortable]Visibility[Sortable]Description[Sortable]Format
Characterization o... .pdf1.84MBLocked until  31/12/2132 Complete Text PDF