Vinsamlegast notið þetta auðkenni þegar þið vitnið til verksins eða tengið í það: http://hdl.handle.net/1946/25669
In this project I studied the effects of glucocorticoid steroids (GCs) on antimicrobial peptide expression and the effects 4-phenyl butyric acid (PBA) and butyric acid have on VDR mediated CAMP expression.
We found that the GC dexamethasone (Dex) suppressed antimicrobial peptide expression in bronchial epithelial cell line and monocyte cell line as well as primary monocytes. This suppression could be countered by 1,25 dihydroxyvitamin D3 (1,25D3) induction, although the induction was still tempered by Dex.
In the other part of the project I found that butyrate and PBA induction of CAMP and HIF1A do not work in the same way, as MAPK (mitogen-activated protein kinase) inhibition had different effects on these inductions. I also showed that an attempted VDR knockout cell line had impaired PBA, butyrate and vitamin D mediated CAMP induction. Computer docking simulations were used to look at the possibility of butyrate and PBA being direct ligands to VDR. According to these simulations butyrate and PBA are weak ligands to VDR although it is still unclear if the chemicals binding is sufficient to activate the receptor.
Skráarnafn | Stærð | Aðgangur | Lýsing | Skráartegund | |
---|---|---|---|---|---|
HIG msritgerðin 03.08.16.pdf | 1.63 MB | Opinn | Heildartexti | Skoða/Opna |