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ThesisUniversity of Iceland>Verkfræði- og náttúruvísindasvið>Meistaraprófsritgerðir>

Please use this identifier to cite or link to this item: http://hdl.handle.net/1946/8412

Title

Innate immunity gene expression in bronchial epithelial cells

Published
May 2011
Abstract

The airway epithelium forms the interface between the external environment and the interior of the human body. The epithelial cells are constantly challenged by potential pathogens and have the crucial function to provide the first line of defense. The epithelial defense strategy consists of both mechanical and chemical components. The mechanical barrier function maintains the integrity of the epithelium to prevent a bacterial breach. The chemical defense strategy consists of the expression and secretion of factors, such as the antimicrobial peptides (AMPs), to prevent the establishment of bacterial infections. The epithelial cells must be able to recognize microbial threats, resulting in the initiation of appropriate immune responses. Here we further characterize the novel bronchial epithelial cell line, VA10, with regard to its receptor expression. Toll-like receptor (TLR) stimulation alone had no effect on AMP expression, while the stimulation together with 4-Phenylbutyrate (4-PBA) and 1,25-Dihydroxyvitamin D3 (1,25D3) indicated an interference with signaling pathways mediating AMP expression. Our findings show that the expression of antimicrobial peptides can be augmented by the stimulation with 4-PBA and 1,25D3. We additionally found that bacterial clearance can be directly induced through stimulation. Together our findings give further insight into the modulating effects of 4-PBA and 1,25D3 on the gene expression in VA10 cells. Both agents aid in the prevention of bacterial infections by augmenting the innate antimicrobial response and acting in anti-inflammatory fashion. These beneficial characteristics further the proposal of 4-PBA and 1,25D3 as novel drug candidates to treat bacterial infections.

Issued Date
10/05/2011


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