Determination of 1-hydroxypyrene in Atlantic cod (Gadus morhua) bile

Abstract

The polyaromatic hydrocarbons (PAH‘s)are the major pollutants related to shipping activities and oil exploitation. On exposure for PAH‘s the absorbed amount is rapidly metabolized to hydrophillic metabolites. These metabolites are important biomarkers for PAH´s exposureIn this work HPLC with fluorescence detection was used in order to measure the concentration of PAH metabolites in Atlantic cod (Gadus morhua) bile from different fishing sites around Iceland and Norway. An enzymatic hydrolysis step before analysis is needed in order to deconjugate the phase II metabolites to hydroxy compounds which can be measured.The 1-hydroxypyrene was detected in all samples,and it has been successfully quantified for Norwegian samples. it can be used as a good bio marker.