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Please use this identifier to cite or link to this item: http://hdl.handle.net/1946/20675

  • A gene near the D3F15S2 site on 3p is expressed in normal human kidney but not or only at a severely reduced level in 11 of 15 primary renal cell carcinomas (RCC)
  • August 1990
  • Renal Cell Carcinoma (RCC) has been associated with the loss of heterozygosity at several loci on the short arm of chromosome 3 (3p). We have previously found that one of these loci, D3F15S2 (pH3H2) was lost in 76% of the tumor cells derived from heterozygous donors (Kovacs, G., Erlandsson, R., Boldog, F., Ingvarsson, S., MüllerBrechlin, R., Klein, G. & Sümegi, J. (1988), Proc. Natl. Acad. Sci., 85, 1571-5). More recently we have identified a putative CpG island in the vicinity of D3F15S2, suggesting that DNA sequences in or around this site may have coding potential (Boldog, F., Erlandsson, R., Klein, G. & Sümegi, J. (1989). Cancer Genet. Cytogenet., 42, 295-306). The screening of a human placenta cDNA library with DNA probes derived from D3F15S2 has led to the isolation of several cDNA clones. They identified a 2.9Kb long message in human placenta and kidney. In total RNA from 11 of 15 primary RCCs the gene expression was reduced to less than 20% compared to eight normal kidneys. This low level of expression may be due to contaminating normal tissue. In the remaining 4 tumors the expression varied from 24-51% compared to normal kidney. To facilitate reference, the gene was provisionally designated as 'RIK'. It was expressed in the HEK 293, one osteosarcoma (HOS), two carcinoma (COLO320 and QDMT), and three Burkitt lymphoma lines (BL2, BL29 and BL31). It was not expressed in one Burkitt lymphoma (DG75) and two EBV transformed lymphoblastoid cell lines (LCL) (NAD-20 and Cherry).

  • RE received stipends from Syskonen Svenssons fond för medicinsk forskning, Robert Lundbergs minnesfond and the Swedish Cancer Society. FB was a recipient of a fellowship from the Swedish Cancer Society. FB, ZM and JS were recipients of fellowships from the Cancer Research Institute and Concern Foundation. JSC was a recipient of a research scholarship granted by the ministry of education, research and universities of the Basque government, Spain. This work was supported by Inga-Britt och Arne Lundbergs Forskningsstiftelse and by National Cancer Institute Grant 5R01CA14054.
  • Oncogene 1990, 5 (8), 1207-1211
  • 0950-9232
  • Mar 16, 2015
  • http://hdl.handle.net/1946/20675

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