Please use this identifier to cite or link to this item: http://hdl.handle.net/1946/21896
Phosphate anions and their derivatives (e.g. phosphorylated proteins, DNA and phospholipids)are widespread in nature and thereof, cis-diol appended phosphate anion derivatives such as adenosine 5’-tri-, 5’-di- and 5’-monophosphate (ATP, ADP and AMP) and cell membrane lipids phosphoinositides (PIs) are ubiquitous. The selective recognition and binding of these biomolecules give insights into numerous biological processes. Therefore, these biomolecules should constitute an important class of targets in the research field of molecular recognition. In spite of this, there is a lack of general tools for the detection and separation of phosphoesters that combine selectivity and sensitivity. Small molecule based chemical receptors show excellent selectivity and sensitivity for phosphate anion derivatives but have limited activity under physiological conditions. Thus, development of biologically active small molecule based chemical receptors is a worthwhile task. This project aims to address this issue by designing and developing a series of novel small molecule based chemical receptors, targeted for the selective recognition and binding of phosphorylated biomolecules such as ATP, ADP, AMP and phosphoinositols. The reported are designed by combing two well-established recognition groups, namely,
a boronic acid moiety; for the selective binding of cis-diol groups (adjacent hydroxyls); and a Zn(II)–2,2’–dipicolylamine complex moiety; for the selective binding of phosphate
groups. Furthermore, fluorophore moiety is also introduced to monitor the binding events by standard spectroscopic methods.