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Vinsamlegast notið þetta auðkenni þegar þið vitnið til verksins eða tengið í það: http://hdl.handle.net/1946/21998

Titill: 
  • Titill er á ensku In vitro analysis of expression vectors for DNA vaccination of horses: the effect of a Kozak sequence
Útgáfa: 
  • Nóvember 2008
Útdráttur: 
  • Útdráttur er á ensku

    One of the prerequisite for developing DNA vaccines for horses are vectors that are efficiently expressed in horse cells. We have analysed the ectopic expression of the human serum albumin gene in primary horse cells from different tissues. The vectors used are of pcDNA and pUC origin and include the cytomegalovirus (CMV) promoter. The pUC vectors contain CMV intron A whereas the pcDNA vectors do not. Insertion of intron A diminished the expression from the pcDNA vectors whereas insertion of a Kozak sequence upstream of the gene in two types of pUC vectors increased significantly the in vitro expression in primary horse cells derived from skin, lung, duodenum and kidney. We report for the first time the significance of full consensus Kozak sequences for protein expression in horse cells in vitro.

Birtist í: 
  • Acta veterinaria Scandinavica, 2008, 50 (1), 44
ISSN: 
  • 0044-605X
Tengd vefslóð: 
  • http://www.actavetscand.com/content/pdf/1751-0147-50-44.pdf
  • http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2600637/pdf/1751-0147-50-44.pdf
  • http://link.springer.com/article/10.1186%2F1751-0147-50-44#page-1
  • http://www.ncbi.nlm.nih.gov/pubmed/18983656
  • https://www.researchgate.net/publication/23451002_In_vitro_analysis_of_expression_vectors_for_DNA_vaccination_of_horses_the_effect_of_a_Kozak_sequence
Samþykkt: 
  • 10.6.2015
URI: 
  • http://hdl.handle.net/1946/21998


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