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Vinsamlegast notið þetta auðkenni þegar þið vitnið til verksins eða tengið í það: http://hdl.handle.net/1946/22014

Titill: 
  • Titill er á ensku Identification of miRNAs in a liver of a human fetus by a modified method
Útgáfa: 
  • Október 2009
Útdráttur: 
  • Útdráttur er á ensku

    BACKGROUND: miRNAs are 17-25 nucleotides long RNA molecules that have been found to regulate gene expression in human cells. There are studies showing that different groups of miRNAs are involved in development of different tissues. In hepatocytes there are reported particular types of miRNAs that regulate gene expression.
    METHODS: We established a human fetal liver cDNA library by a modified cloning protocol. Then plasmid isolation from the colonies was performed. After sequencing and database searching, the miRNAs were recognized. RT-PCR and sequencing were carried out to validate the miRNAs detected. Real-time PCR was used to analyze the expression of each miRNA.
    RESULTS: One novel miRNA was discovered, together with another 35 previously-known miRNAs in the fetal liver. Some of them existed in variants. The miRNAs identified were validated by RT-PCR and sequencing. Quantitative analysis showed that they have variable expression.
    CONCLUSION: Our results indicate that a special group of miRNAs may play an important role in fetal liver development in a synergistic manner.

Styrktaraðili: 
  • Styrktaraðili er á ensku The Huazhong University of Science and Technology (24510101 and 25510026)
Birtist í: 
  • Plos One 4(10), 2009
ISSN: 
  • 1932-6203
Tengd vefslóð: 
  • http://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0007594&representation=PDF
  • http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2762743/pdf/pone.0007594.pdf
  • http://web.a.ebscohost.com/ehost/pdfviewer/pdfviewer?vid=1&sid=f96244af-0de5-47a2-b86b-a5ca4bd81bf2%40sessionmgr4004&hid=4106
  • http://www.ncbi.nlm.nih.gov/pubmed/19855840
  • https://www.researchgate.net/publication/38035253_Identification_of_miRNAs_in_a_liver_of_a_human_fetus_by_a_modified_method
Samþykkt: 
  • 11.6.2015
URI: 
  • http://hdl.handle.net/1946/22014


Skrár
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