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Vinsamlegast notið þetta auðkenni þegar þið vitnið til verksins eða tengið í það: http://hdl.handle.net/1946/22348

Titill: 
  • Titill er á ensku Demethylation of the region around exon 2 of MLH1 gene in gastrointestinal cancer
Útgáfa: 
  • Nóvember 2012
Útdráttur: 
  • Útdráttur er á ensku

    Aim: Our previous study showed that methylation of the mutL homolog 1 (MLH1) promoter may spread upstream from the Alu elements in intron 1. In this study, we investigated if the Alu methylation could also spread downstream. Materials and Methods: Two colorectal cancer cell lines (RKO, SW48), and four colorectal and three gastric carcinomas [all Microsatellite Instability (MSI)-positive] were selected as cases. Normal colorectal and gastric mucosa and human peripheral blood, and one MSI-negative colorectal cancer case served as controls. After extraction of DNA, bisulfite genomic sequencing was used to analyze the methylation level of exon 2 and the adjacent part of intron 2 of the MLH1 gene. Results: Exon 2 and the partial intron 2 exhibited a high level of methylation in controls. In contrast, demethylation in these regions was seen in gastrointestinal cancer. Conclusion: Exon 2 methylation is not likely to influence MLH1 gene expression. It seems that de-methylation of exon 2 and intron 2, in combination with intron 1, is associated with methylation spreading of the MLH1 promoter. The region around exon 2 could possibly bind proteins that regulate methylation, and therefore affect gene expression.

Birtist í: 
  • Anticancer Research, 2012, 32, 4861-4864
ISSN: 
  • 0250-7005
Tengd vefslóð: 
  • http://ar.iiarjournals.org/content/32/11/4861.full.pdf+html
  • http://www.ncbi.nlm.nih.gov/pubmed/23155252
  • https://www.researchgate.net/publication/233424308_Demethylation_of_the_Region_around_Exon_2_of_MLH1_Gene_in_Gastrointestinal_Cancer
Samþykkt: 
  • 13.7.2015
URI: 
  • http://hdl.handle.net/1946/22348


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