Vinsamlegast notið þetta auðkenni þegar þið vitnið til verksins eða tengið í það: http://hdl.handle.net/1946/29847
Today, one of the biggest challenges in breast cancer treatment is metastasis formation in other organs, such as the lungs. Development of models that mimic the biomolecular environment of metastatic breast cancers can help metastasis research and eventually lead to the discovery of genes that can be used for further development of breast cancer treatment in the future. Since the E0771 cell line in genomically unstable it shows the ability to metastasize to various organs of different extent. By establishing a lung specific subline of the E0771 cell line, genes involved only with lung metastasis can be discovered.
The aim of this research was to isolate a specific E0771 breast cancer subline with high organotropism to the lungs that could be used to identify key steps in the metastatic progression to the lungs.
The E0771 cell line, a luminal A breast cancer cell line with metastatic ability to the lungs was used. A specified subline was isolated by using in vivo image-guided screening method. In this method, E0771 parental cells with constitutive expression of firefly luciferase were injected via tail vein into C57BL/6 mice and cells metastasized and proliferated in the lung were harvested following the guide of bioluminescence imaging. After three rounds of in vivo image-guided screening, the obtained E0771 subline was evaluated with in vitro assays.
In vivo results showed significantly higher growth signals in lungs of the established E0771 subline compared to the parental E0771 cell line. Additonally, metastatic formation of the subline was limited to the lungs while tropism of the parental line was nonspecific.
The isolation of a specific E0771 subline with high tropism to the lungs was successful resulting a novel lung metastatic breast cancer model. The future use of this model could provide key findings regarding metastatic behavior of luminal A breast cancers and thus aid in the discovery of new cancer treatments.
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