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Vinsamlegast notið þetta auðkenni þegar þið vitnið til verksins eða tengið í það: http://hdl.handle.net/1946/35469

Titill: 
  • Titill er á ensku Pre-validation of UPLC-MS/MS method for 15- steroid hormone panel measurement - With design of experiments (DoE)
Námsstig: 
  • Diplóma meistara
Efnisorð: 
Útdráttur: 
  • Útdráttur er á ensku

    Steroid hormones are synthesized from cholesterol and perform numerous physiological functions.
    Steroid hormones are synthesized in the adrenal glands, the gonads and during pregnancy in the placenta and can be classified into five groups depending on the type of receptor they bind to, mineralocorticoids, glucocorticoids, androgens, estrogens and progestins.
    Measurements of steroid hormones in serum and urine play an important role in the clinical evaluation of a number of endocrine disorders like congenital adrenal hyperplasia. Accurate and precise analytical methods are therefore vital.
    Advantages of LC-MS/MS measurements in addition to increased specificity and sensitivity is the ability to measure simultaneously multiple analytes from a small volume sample, which is especially valuable when dealing with neonatal samples.
    The aim of the research project was to add thirteen steroid hormones to the existing SPH method, establishing a steroid hormone profile on LC-MS/MS that will include steroid hormone precursors that will assist in diagnostic and treatment of CAH as well as other diseases, get baseline separation of critical pairs of steroid isomers, including 11-deoxycortisol, corticosterone, 21-deoxycortisol, 17-OHP and DHEA, optimize the chromatography of the designed method using Design of Experiments (DoE).
    Because of unforeseen situations in the society, Covid-19 and union Efling strike, we were not able to finish the practical part. A baseline separation of the peaks in question was achieved with the help of DoE and that work can be used to continue and advance the research project.

Samþykkt: 
  • 20.5.2020
URI: 
  • http://hdl.handle.net/1946/35469


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