Antimicrobial coating for silicone medical devices: Development of the activation procedures, ninhydrin and fluorescence assays.

Abstract

The development of antimicrobial coatings for silicone medical devices requires a reliable method for surface activation and functionalization. This study investigated various strategies for modifying silicone surfaces to enable stable antimicrobial coatings using click chemistry and other approaches. Multiple activation methods were explored.. The study also assessed the impact of solvent choice, reaction time, and surface preparation on functionalization efficiency, as determined by fluorescence and FTIR spectroscopy. Key findings indicated that while APTMS enhances surface reactivity, it also compromises the structural integrity of silicone, whereas EA treatment offered a more controlled modification. Click chemistry with azidomethyloxirane successfully introduced azide groups, enabling further functionalization. Antimicrobial assays demonstrated the potential of these coatings in preventing bacterial adhesion. These insights contribute to the advancement of biocompatible, antimicrobial silicone coatings for medical applications.